PHAR 7632 Spring 1999
Biopharmaceutics
OU HSC College of Pharmacy
First Semester Exam
18 February 1999
Section ONE. Sketch a Graph or Diagram Sketch the graphs or diagrams requested in the space provided. Include any distinguishing characteristics. Assume that a linear one compartment model applies unless otherwise specified. Include labels and units. 4 x 4 = 16 points
Q 1.1 Sketch the components of the GLC assay method. Label three components.
2 1/2" X 2 1/2" box for answer.
Q 1.2 Linear plot of Cp versus time after an IV Bolus Dose.
2 1/2" X 2 1/2" box for answer.
Q 1.3 Sketch the components of the EMIT assay method. Label three components.
2 1/2" X 2 1/2" box for answer.
Q 1.4 A semi-log plot of Cp versus time after an IV Bolus Dose.
2 1/2" X 2 1/2" box for answer.
Section TWO. True/False Check the Correct Response. 10 x 2 = 20 points
Q 2.1 The value of e-0.58 is 1.786.
Q 2.2 Detectors used for quantitation with the HPLC method include electron capture and flame ionization.
Q 2.3 The EMIT assay method involves quantitation using the measurement of radioactivity associated with the bound drug fraction.
Q 2.4 The point-slope method can be used to numerically integrate differential equations.
Q 2.5 The Laplace transform technique can be used to numerically integrate differential equations.
Q 2.6 The Laplace of a constant is the constant divided by s.
Q 2.7 Boomer can be used to calculate concentration versus time data using given parameter values or to find best values for parameters.
Q 2.8 When a substance emits light by fluorescence it is usually at the same wavelength as the excitation wavelength.
Q 2.9 Spreadsheets are useful for calculating and graphing various equations related to pharmacokinetics.
Q 2.10 UV-visible absorption spectra exhibit sharp peaks because the absorption of light involves only electronic transitions and no transfer of energy via rotation or vibration.
Section THREE Short Answer 3 x 5 = 15 points
Remember to limit the time spent on this section.
Q 3.1 Briefly describe how the point-slope method is used to solve differential equations
6 1/2" X 2 1/2" box for answer.
Q 3.2 Describe Beer's law
6 1/2" X 2 1/2" box for answer.
Q 3.3 Describe the RIA assay method
6 1/2" X 2 1/2" box for answer.
Section FOUR Calculations This section = 49 points
Show all your work for full credit. All material not deleted or crossed-out will be considered for grading. Put labels and units on all requested graphs.
Q 4.1 (24 points) A 300 mg dose of a drug was given by IV bolus injection and the data below were collected. Plot the data on semi-log graph paper, draw the ‘best’ line and determine Cp0, kel, and total AUC using the trapezoidal rule.
|
Time (hr) |
Concentration (mg/L) |
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|
1 |
13.5 |
|||
|
2 |
10.2 |
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|
4 |
8.0 |
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|
8 |
3.6 |
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12 |
2.2 |
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14 |
1.4 |
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Q 4.2 (25 points) Determine the analytical equation for X2 as a function of time using the Laplace transform method. The dose is put into the GI, component 1, at time zero. The two rate constants, ka and kel, are first order.
The diagram is shown below: