Pharmaceutical Analysis
Student Objectives for this Chapter
- Describe the need and techniques for separatrion of a drug from the experimental matrix
- Describe methods use to quantitate drug concentrations
- Understand the Advantages and Disadvantages of some these methods
Topics to be covered in this chapter
- Spectroscopic Analysis
- Chromatographic Separation
- Sample Preparation
- High Performance (Pressure) Liquid Chromatography (HPLC)
- Gas Liquid Chromatography (GLC)
- Radioimmunoassay (RIA)
- Enzyme Multiplied Immunoassay (EMIT)
- Fluorescence Polarization Immunoassay (FPIA)
- Comparison of Clinical Analytical Assay Methods
Pharmaceutical Analysis generally involves two steps; a) separation of the compound of interest and b) quantitation of the compound. Often, the better the separation the easier the quantitation.
References
- Bauer, H.H., Christian, G.D., and O'Reilly, J.E. Instrumental Analysis, Allyn and Bacon, Boston, (1978) ISBN 0-205-06556-2
- Connors, K.A., A Textbook of Pharmaceutical Analysis, 2nd edition, Wiley-Interscience, New York (1975) ISBN 0-471-16853-X
- Higuchi, T. and Brochmann-Hanssen, E. Pharmaceutical Analysis, Interscience, New York, (1961)
- Kolthoff, I.M., Sandell, E.B., Meehan, E.J., and Bruckenstein, S. Quantitative Chemical Analysis, Macmillan Company, New York, (1969)
- Pieper, J.A. and Rutledge, D.R. Current Concepts - Laboratory Techniques for Pharmacists, Upjohn, Kalamazoo, MI (1989)
- Smith, R.V., and Stewart, J.T. Textbook of Biopharmaceutic Analysis, Lea and Febiger, Philadelphia (1981) ISBN 0-8121-0770-5
- Search for Pharmaceutical Analysis at Goggle
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Copyright 2001-3 David W. A. Bourne (david@boomer.org)
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Thursday 30 Jan 2003 at 12:31 PM