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Relative affinity may be based on relative solubility, adsorption, size or charge. Differences in solubility are expressed by partitioning between the mobile and stationary phases. Adsorption differences cause the separation of molecules in a non aqueous environment. Permeation (gel permeation) chromatography is based on smaller molecules being retained by inclusion without smaller pores of the gel. Separation by ion-exchange chromatography is based on the exchange of ions in the mobile phase with ions on the stationary phase. As such it is better suited to purification than separation between similar materials.
Commonly used chromatography methods are high performance/pressure liquid chromatography (HPLC), gas liquid chromatography (GLC) and thin layer chromatography (TLC).
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click the arrow to start the chromatography |
Figure 3.3.1 Column chromatography with a multicomponent sample
Copyright 2001-3 David W. A. Bourne (david@boomer.org)