Gas Liquid Chromatography (GLC)

GLC Instrumentation

• Gas cylinder and regulator
  • Nitrogen
  • Helium
  • Argon
  • Hydrogen
• Injection port
• Column
  • Gas-solid - adsorption
  • Gas-liquid - High boiling point stationary phase
  • Glass, steel, capillary glass
• Column oven
• Detector
  • Flame ionization (FID) general purpose - modest sensitivity
  • Nitrogen phosphorus FID specific for N and P
  • Electron capture (EC)
  • Mass spectrometry (MS)
• Recorder

Fig 3.6.1 Diagram illustrating a Typical GLC System
Redrawn from: Pieper and Rutledge, Laboratory Techniques for Pharmacists, Upjohn 1989, page 20, figure 2

Fig 3.6.2 Steel column installed in Oven

Fig 3.6.3 Typical chromatogram

• Gas Chromatography

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Copyright 2001-3 David W. A. Bourne (david@boomer.org)