PharmPK Discussion - Amidase and Phosphatase Inhibitor in Blood

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On 15 Nov 2007 at 17:23:36, Julius Apuy (chmstryaggie.-a-.yahoo.com) sent the message


The following message was posted to: PharmPK

To all PharmPk readers,

I found some inhibitor and inhibitor cocktail for
amidases and phosphatases, however, it is as applied
to cell lysates.

Has anyone have an experience in inhibiting
phosphatases and amidases in blood and plasma samples.

I tried Calbiochem's Phosphatase Inhibitor cocktail
which includes:

Imidazole, NaF, Na3MO4, Na3VO4,Na Tartrate.  at around
1-5mM final conc in plasma and blood.  But that doesnt
seem to work.

For the amidase inhibitor, Im trying PMSF for now.

Advice on this matter is welcome.

JLA

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On 16 Nov 2007 at 12:38:11, "Kishore Khan" (kishore0123.-a-.gmail.com) sent the message


We have tried Sigma Phosphotase Cocktail Inhibitor 2 for one of our
assays to inhibit phosphotases in plasma and it has worked fine so far.

Thanks,

Kishore

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