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Radioimmunoassay (RIA)

RIA involves the separation of the drug using the specificity of antibody - antigen binding and quantitation using radioactivity.

Components of RIA Assay Kit

General Procedure for Performing a RIA Analysis

A Blank and Three Standard Samples

RIA Assay Schema

Fig 3.7.1 RIA before and after Incubation - Blank and Three Standard Samples

Table 3.7.1 Bound and Free Drug Concentrations

Total [Drug] Bound [Drug] Free [Drug]
0 6 0
3 4 2
6 3 3
12 2 4

Plot of Bound versus Total Drug Concentration

Fig 3.7.2 Plot of Bound versus Total Drug Concentration

Logit versus Log C Plot

Fig 3.7.3 Logit versus Log Total C Plot


References

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Copyright 2001-3 David W. A. Bourne (david@boomer.org)


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