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I search a HPLC method to analyse in serum, an antibiotic,
cephalosporin = ceftazidime.
Could you help me to find it, please ?
Thank you for your help.
Laurence MANGIN
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Hi Laurence,
You may take a look at the following two papers.
Good luck,
Edward Lai
SIMULTANEOUS LIQUID-CHROMATOGRAPHIC ANALYSIS OF THE BETA-LACTAM ANTIBIOTICS
CEFAZOLIN, CEFADROXIL, CEPHALEXIN, AMPICILLIN, AND CEPHRADINE IN SOLUTION/
FARAG SA
IN: JOURNAL OF AOAC INTERNATIONAL 1998 MAR-APR Vol.81 No.2 Pages 381 - 385
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A liquid chromatographic method was developed for the determination of
nanogram quantities of 5 broad-spectrum structurally related beta-lactam
antibiotics (cefazolin, cefadroxil, cephalexin, cephradine, and ampicillin)
in solution. The method uses a C-18 reversed-phase column, UV absorption
(240 nm) detection, and an aqueous mobile phase containing isopropyl alcohol
and acetic acid. Relative resolution between the antibiotic peaks ranged
from 1.7 to 5.9 for all peaks. Chromatographic retention times were 2.97,
3.92, 4.57, 5.37, and 6.56 min for cefazolin, cefadroxil, cephalexin,
ampicillin, and cephradine, respectively. Accuracy, precision, linearity,
and long term analytical reproducibility were determined by statistical
analysis. Use of the proposed method to evaluate the degradation of
cephradine solutions stored at room temperature illustrated its potential as
a stability-indicating assay.
MULTIDIMENSIONAL EVALUATION OF IMPURITY PROFILES FOR GENERIC CEPHALEXIN AND
CEFACLOR ANTIBIOTICS/
OLSEN BA, BAERTSCHI SW, RIGGIN RM,
IN: JOURNAL OF CHROMATOGRAPHY 1993 OCT 1 Vol.648 No.1 Pages 165 - 173
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QD271.A1J615
FLOOR 1 SER: v.652-755,n.2, 1993-96 cancelled 1996
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A multidimensional approach is described for characterizing impurities in
samples of generic cefaclor and cephalexin antibiotics. High-performance
liquid chromatography (HPLC) with gradient elution followed by photodiode
array or mass spectrometric detection provides valuable information
concerning the nature of impurities observed. Results are presented which
demonstrate the utility of these techniques for identifying impurities and
distinguishing among process-related impurities, degradation products and
formulation excipients. Preparative HPLC isolation and spectroscopic
identification of some impurities is also described.
PharmPC Discussion List Archive Index page
David Bourne (david@boomer.org)