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Dear all,
we are tryng to dose ropivacaine in children after central block.
Unfortunately our technicins are not able to do that, they have write.....
......................" Blood samples were collected in heparinised
Venoject tubes with green stoppers. The blood was centrifuged and the plasma
was stored at -20=B0C until analysis.
Two standard solutions (about 3mg/ml) of ropivacaine and penticaine were
prepared. After addition of 300ng of ropivacaine and penticaine, the
drug-free plasma (0.5ml) was adjusted to pH 9.2 by the addition of 0.5 ml 2%
sodium tetraborate, and the analytes were extracted into 10 ml diethylether
by shaking vigorously for 5 min. After centrifugation (3000rpm for 5 min)
the organic phase was back-extracted into 0.2 ml 0.1 M H2SO4 by shaking well
for 1 min. After discarding the organic phase, aliquots of the acid were
injected into the HPLC. The HPCL system consisted of Merck Li ChroCART
250-4 LiChromopher 100 RP-18 (5?m) and a solvent of 25% acetonitrile in 45
mM phosphate buffer pH 3. Eluiting compounds were detected by absorbance at
220 nm, flow 1.5 ml/min.
But it don't work
Could someone help us
thank in advance,
Dott Marco Calamandrei
Pediatric hospital "A. Meyer" Firenze Italia
mc8344.at.mclink.it
PharmPC Discussion List Archive Index page
David Bourne (david@boomer.org)