PharmPC Discussion - Caco-2

• On 3 Aug 1998 at 12:53:40, Richard Prankerd (richard.prankerd.at.pharmacy.uq.edu.au) sent the message
  

  
  Colleagues:
  
  Has anyone performed or encountered studies which suggest that Caco-2 cell
  lines are somewhat "leaky", such that drug permeation is not fully
  controlled by active or passive transmembrane processes?
  
  Please pass this enquiry on to others who you know may have an answer,
  Thanks in advance
  Richard
  
  Richard J. Prankerd, PhD
  Senior Lecturer
  School of Pharmacy
  University of Queensland        Phone: INT + (617) 3365-3179
  St Lucia QLD 4072               Fax: INT + (617) 3365-1688
  AUSTRALIA               richard.at.uqpharmacy.pharmacy.uq.edu.au
                          http://www.uq.edu.au/pharmacy/academic.html
  
  

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• On 11 Aug 1998 at 15:29:53, (David_Perng-1.at.sbphrd.com) sent the message
  

  
  David,
  
  This depends on culture conditions (e.g. days, seeding density, passage,
  etc).  Bit I think there are enough evidences showing that Caco-2 are not
  leaky and could be used for drug transport study.  In terms of active
  transport or metabolic activities, caco-2 may be underexpressed for these
  systems.  An recent review article about Caco-2 is by Florence Delie and
  Werner Rubas in Critical Rev. in Ther. Drug Delivery System 14:221-286
  (1997).
  
  If you would like to discuss more, please give me a call.
  
  Cheers,
  
  Ping
  
  

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David Bourne (david@boomer.org)