PharmPC Discussion - BAC analysis

• On 12 Apr 1999 at 22:26:55, "JRLOPEZ" (jr_lopez.at.jet.es) sent the message
  

  
  Dear colleagues,
  
  We're working in the HPLC analysis of Benzalkonium Chloride in ophthalmic
  solutions containing polyethyleneglycol. So far we have tried Cyano and
  Phenyl stationary phases, using different mobile phases (TEA:MeCN; Na
  heptanesulphonate buffered with phosphate:MeCN) and we have not succeded
  yet in fully separating BAC from the matrix of the formula.
  
  Should anybody has a solution, please contact us.
  
  Thanks in advance :-)
  
  

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• On 13 Apr 1999 at 23:12:12, Richard Prankerd (richard.prankerd.at.pharmacy.uq.edu.au) sent the message
  

  
  In reply to the following question....
  
  Was there any reason for not using a standard C18 column? I would have
  thought that this would be more discriminating between a polar matrix
  material such as PEG and a surfactant hydrocarbon tail, rather than cyano
  (which is quite polar). I can see the rationale for using a phenyl column,
  but these are expensive and fragile, and would almost always try a C18 (or
  possibly C8) first.
  
  Regards
  Richard
  
  Richard J. Prankerd, PhD
  Senior Lecturer
  School of Pharmacy
  University of Queensland        Phone: INT + (617) 3365-3179
  St Lucia QLD 4072               Fax: INT + (617) 3365-1688
  AUSTRALIA               richard.at.pharmacy.uq.edu.au
                          http://www.uq.edu.au/pharmacy/rprank.html
  (including photo)
  
  

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David Bourne (david@boomer.org)