PharmPC Discussion - Characterization of insulin drug conjugate

• On 7 Dec 1998 at 16:51:03, paranjpe.at.buffalo.edu sent the message
  

  
  hi,
   i am working in the area of enhanced drug delivery thru' BBB using
  insulin fragments as delivery enhancers. my problem is how to characterize
  the insulin-drug conjugate, other than chemical characterization. i am
  specifically interested in physical methods like XRD and DSC etc.
  kindly suggest other possible modes to define the prepared conjugate,
  including literature .
  thanx
  pankaj
  
                               Pankaj V. Paranjpe
  
  131 Biomedical Education Bldg.                  121 Heath Street
  School of Medicine and Biomedical Sciences      Buffalo, NY 14214
  State University of New York at Buffalo         Ph: (716) 838 8482
  Buffalo, NY 14214
  
  

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• On 8 Dec 1998 at 12:10:05, "Richard Prankerd" (rprankerd.at.hotmail.com) sent the message
  

  
  Pankaj:
  
  XRD and DSC are normally very useful solid phase techniques for
  interaction studies (although I have my doubts about DSC measurements on
  peptides, due to their thermal instability). Suggest you also consider
  isothermal reaction calorimetry (either batch mode or flow; solution
  interactions) infra-red spectroscopy (solid and solution interactions)
  and NMR nuclear Overhauser effect enhancement (solution interactions).
  Depending on the size of the fragments and availability of material, you
  may see some effects from careful viscosity measurements. Other
  possibilities include circular dichroism or optical rotatory dispersion,
  dielectric spectroscopy and ligand concentration dependent shifts of UV
  absorption or fluorescence bands.
  
  regards
  Richard
  
  

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