PharmPC Discussion - Spectrophotometric method to calculate the stability constant

• On 29 Apr 1999 at 23:01:06, "Michaleas Sotiris" (smich.at.aias.net) sent the message
  

  
  I wonder if there is a spectrophotometric method to calculate the stability
  constant of a 1:2 complex iron+2ligands) . Talking about a complex with a
  lambda max at 569
  Any ideas or references
  Thank you
  Michaleas Sotiris
  PhD student
  
  

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• On 1 May 1999 at 19:14:11, Richard Prankerd (richard.prankerd.at.pharmacy.uq.edu.au) sent the message
  

  
  To PharmPC list:
  
  The use of spectrophotmetry to determine multiple stability constants is
  fully discussed in Ch. 4 of Connors, KA, Binding Constants, Wiley
  Interscience New York (1987). In this chapter, Connors states that
  spectrophotometry "is not in general a very powerful means for studying
  multiple equilibria". He recommends that non-linear regression be used to
  evaluate the changes in absorbance for various concentrations that arise
  due to the presence of other than simple 1:1 equilibria, rather than any
  other approach.
  
  Regards
  Richard
  
  Richard J. Prankerd, PhD
  Senior Lecturer
  School of Pharmacy
  University of Queensland        Phone: INT + (617) 3365-3179
  St Lucia QLD 4072               Fax: INT + (617) 3365-1688
  AUSTRALIA               richard.at.pharmacy.uq.edu.au
                          http://www.uq.edu.au/pharmacy/rprank.html
  
  

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David Bourne (david@boomer.org)