Assay Methods

Assays are usually under the direction of the pathology or clinical chemistry laboratory [1].

Methods: GLC, HPLC, RIA, EMIT, fluoroimmunoassay, luminescence

Criteria: Speed (turn-around in 2-3 hours or less), good accuracy within the therapeutic range for drug studied.

HPLC (Reference 29, p26-33)

Components of the Instrument

• Pump
• Injector
• Column
  • Normal Phase
  • Reverse Phase
  • Ion-Exchange Column
• Detector
• Recorder
• Quantitation
  • Peak Height/Area
  • External/Internal Standard

GLC (Reference 29, p19-22)

Components of the Instrument

• Gas cylinder and Regulators
• Injection port
• Column/Column Oven
• Detector
• Recorder

RIA (Reference 29, p36 - 39)

Components of the Assay

• Antibody
• Labeled Drug
• Radioactivity Measurement
• Quantitation

EMIT (Reference 29, p40 - 42)

Components of the Assay

• Antibody
  
• Substrate
  
• Enzyme
  
• Quantitation

Copyright 2001 David W.A. Bourne