Chapter 23
Pharmaceutical Analysis
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Student Objectives for this Chapter
- Describe the need and techniques for separation of a drug from the experimental matrix
- Describe methods use to quantitate drug concentrations
- Understand the Advantages and Disadvantages of some these methods
Topics to be covered in this chapter
- Sample Preparation
- Spectroscopic Analysis
- Chromatographic Separation
- High Performance (Pressure) Liquid Chromatography (HPLC)
- Gas Liquid Chromatography (GLC)
- Radioimmunoassay (RIA)
- Enzyme Multiplied Immunoassay (EMIT)
- Fluorescence Polarization Immunoassay (FPIA)
- Comparison of Clinical Analytical Assay Methods
Pharmaceutical Analysis generally involves two steps; a) separation of the compound of interest and b) quantitation of the compound. The better the separation the easier the quantitation.
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