Chapter 24

Pharmaceutical Analysis

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Radioimmunoassay (RIA)

RIA involves the separation of the drug using the specificity of antibody - antigen binding and quantitation using radioactivity.

Components of RIA Assay Kit

General Procedure for Performing a RIA Analysis

A Blank and Three Standard Samples

RIA Assay Schema

Fig 3.7.1 RIA before and after Incubation - Blank and Three Standard Samples

Table 24.7.1 Bound and Free Drug Concentrations
Drug Bound Y'
B/Bo
Labeled
Drug, B
Drug
0 6 (Bo) 0 -
3 4 2 0.667
6 3 3 0.5
12 2 4 0.333
30 1 5 0.167

Plot of Bound versus Total Drug Concentration

Fig 24.7.2 Plot of Bound versus Total Drug Concentration

Logit versus Log C Plot

Fig 24.7.3 Logit versus Log Total C Plot


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