Chapter 24

Pharmaceutical Analysis

return to the Course index
previous | next

Comparison of Clinical Assay Methods

Basis for Comparison of Methods

Table 24.10.1 Comparison of Assay Methods
  HPLC GLC RIA EMIT FPIA
Separation Method Chromatography Chromatography Immunoassay Immunoassay Immunoassay
Quantitation Method Various including absorption, fluorescence, electrochemical, radioactivity, refractive index Various including flame ionization, thermal conductivity, electron capture, mass spectrometry Radioactivity Kinetic via Absorption Fluorescence
Sample Size (µl) 100 - 500 100 - 500 100 50 50
Sample Type* P, S, U, Sa P, S, U, Sa P, S, U, Sa P, S, U P, S, U
Time (hr) 0.5 0.5 0.25 0.1 - 0.3 0.1 - 0.2
Sensitivity** 1 1 2 3 2
Specificity** 1 1 4 4 3
Accuracy** 2 2 2 2 2
Precision** 3 3 2 1 1
Training** 5 5 3 3 2
Equipment Cost** 4 4 5 3 4
Reagent Cost** 3 2 2 3 2
Clinical Application** 4 5 4 2 1
Research Application** 1 2 3 3 3

* Plasma, Serum, Urine, Saliva
** 1 excellent - 5 poor
Redrawn from: Pieper and Rutledge, Laboratory Techniques for Pharmacists, Upjohn 1989, page 47, table 3

Student Objectives for this Chapter

return to the Course index


This page was last modified: Wednesday, 18th Oct 2017 at 8:15 pm


Privacy Statement - 25 May 2018

Material on this website should be used for Educational or Self-Study Purposes Only

iBook and pdf versions of this material and other PK material is available

Copyright © 2001-2021 David W. A. Bourne (david@boomer.org)